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平臺編號：bio-69400
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國際編號：CCL-85?
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細(xì)胞信息： EB-3
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
EB-3
年限：3 years
細(xì)胞類型：B淋巴細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CCL-85?
相關(guān)疾?。毫馨土?br /> 運輸方式：凍存運輸
細(xì)胞形態(tài)：淋巴樣
生長狀態(tài)：懸浮生長
規(guī)格：50次 Designations: EB-3 [EB3]
Depositors: ?W Henle
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:partially resistant to polioviruses and vesicular stomatitis virus
Antigen Expression:HLA A3, Aw32, Cw2
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
Isoenzymes: G6PD, A
Age: 3 years
Gender: male
Ethnicity: Black
Comments:EBNA positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
26251: . . Trans. N.Y. Acad. Sci. 29: 71, 1966.
26252: . . Wistar Inst. Symp. Monogr. 4: 59, 1965.
細(xì)胞類型：B淋巴細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CCL-85?
相關(guān)疾?。毫馨土?br /> 運輸方式：凍存運輸
細(xì)胞形態(tài)：淋巴樣
生長狀態(tài)：懸浮生長
規(guī)格：50次 Designations: EB-3 [EB3]
Depositors: ?W Henle
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:partially resistant to polioviruses and vesicular stomatitis virus
Antigen Expression:HLA A3, Aw32, Cw2
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19
Isoenzymes: G6PD, A
Age: 3 years
Gender: male
Ethnicity: Black
Comments:EBNA positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 3 X 10 exp5 viable cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 21415: Henle G, et al. Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc. Natl. Acad. Sci. USA 59: 94-101, 1968. PubMed: 5242134
22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
26251: . . Trans. N.Y. Acad. Sci. 29: 71, 1966.
26252: . . Wistar Inst. Symp. Monogr. 4: 59, 1965.
