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平臺編號：bio-69402
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國際編號：CRL-2261?
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細胞信息： RL
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
RL
細胞類型：B淋巴細胞
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：1
物種來源：人
ATCC Number：CRL-2261?
相關疾?。浩渌膊?br /> 運輸方式：凍存運輸
數(shù)量：大量
年限：52 years
細胞形態(tài)：淋巴樣
免疫類型：IgM; lambda light chain
組織來源：ascites
規(guī)格：30 ml Designations: RL
Depositors: ?M Beckwith
Isotype: IgM; lambda light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: non-Hodgkin's lymphoma
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Cytogenetic Analysis:the cells possess a 14:18 translocation
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments:RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
RL cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
生長狀態(tài)：懸浮生長
是否是腫瘤細胞：1
物種來源：人
ATCC Number：CRL-2261?
相關疾?。浩渌膊?br /> 運輸方式：凍存運輸
數(shù)量：大量
年限：52 years
細胞形態(tài)：淋巴樣
免疫類型：IgM; lambda light chain
組織來源：ascites
規(guī)格：30 ml Designations: RL
Depositors: ?M Beckwith
Isotype: IgM; lambda light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: ascites
Disease: non-Hodgkin's lymphoma
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin; surface IgM and IgD; secreted IgM (low levels)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,11
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9,10
TPOX: 9,11
vWA: 19,20
Cytogenetic Analysis:the cells possess a 14:18 translocation
Age: 52 years
Gender: male
Ethnicity: Caucasian
Comments:RL is a human non-Hodgkin's lymphoma B cell line originated in 1983 by Walter J. Urba and Dan L. Longo.
Exposure of RL cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
RL cells have been reported to be Epstein-Barr virus genome negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
