數(shù)量:大量
是否是腫瘤細(xì)胞:1
物種來源:人
生長狀態(tài):貼壁生長
細(xì)胞形態(tài):其他
ATCC Number:CRL-7724?
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年限:60 years
運(yùn)輸方式:凍存運(yùn)輸
器官來源:皮膚
規(guī)格:100 ul Designations: SH-4
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixture of spindle-shaped and epithelial-like cells
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: pleural effusion
Cellular Products:melanin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 11,12
D5S818: 11,12
D7S820: 13
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Cytogenetic Analysis:modal number = 53; range = 45 to 56
Age: 60 years
Gender: female
Ethnicity: Caucasian
Comments:Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
References: 22395: Seman G, et al. Establishment of a cell line (SH-4) from pleural effusion of a patient with melanoma. In Vitro 11: 205-211, 1975. PubMed: 1176156