數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
運(yùn)輸方式:凍存運(yùn)輸
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
ATCC Number:CCL-87?
相關(guān)疾?。毫馨土?br />
細(xì)胞類型:B淋巴細(xì)胞
是否是腫瘤細(xì)胞:1
物種來源:人
年限:7 years
規(guī)格:60 次                                                                                                                                                                                                                                                                                                                                                          Designations: Jiyoye [Jijoye, P-2003, P-3J]
Depositors:                  ?W Henle 
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped:        frozen 
Medium & Serum: See Propagation          Growth        Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease:  Burkitt's lymphoma 
Cell Type:  B lymphocyte;
Cellular Products:Epstein-Barr virus (EBV)
Permits/Forms:In addition to the MTA          mentioned above, other ATCC            and/or regulatory permits  may be required for the          transfer of this ATCC material. Anyone purchasing ATCC          material is ultimately responsible for obtaining the permits.          Please click here  for information regarding          the specific requirements for shipment to your location.
Applications:The cells carry and produce          Epstein-Barr virus (EBV).
Virus        Resistance:The cells are partially resistant to          poliovirus and vesicular stomatitis viruses.
Antigen        Expression:HLA A32, B17, Bw37
DNA          Profile (STR):Amelogenin: X,Y 
CSF1PO: 10,11 
D13S317: 12 
D16S539: 10,11 
D5S818: 12 
D7S820: 8,10 
THO1: 7,9 
TPOX: 6,8 
vWA: 15,19
Isoenzymes:        G6PD, B
Age:        7 years 
Gender:        male 
Ethnicity:        Black, African 
Comments:The cells carry and produce          Epstein-Barr virus (EBV). 
EBNA positive.
Propagation:  ATCC complete growth medium:  The base          medium for this cell line is ATCC-formulated RPMI-1640 Medium,          Catalog No. 30-2001.  To make the complete growth medium, add          the following components to the base medium:  fetal bovine          serum to a final concentration of 10%.
Temperature:  37.0°C 
Atmosphere:  air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal:  Add fresh medium every 2 to          3 days (depending on cell density) 
Cultures can be          maintained by the addition of fresh medium or replacement of          medium. Alternatively, cultures can be established by          centrifugation with subsequent resuspension at 4 to 6 X 10          exp5 viable cells/ml. 
A maximum of 2 - 3 X 10 exp6          viable cells/ml is obtainable.
Preservation:        culture medium 95%; DMSO, 5%
Related        Products:Recommended medium (without the          additional supplements or serum described under ATCC          Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1484
derivative:ATCC HTB-62
References:           22246: . .  J. Clin. Pathol. 38: 261, 1985.
26255: Kohn G, et al. Involvement of C          group chromosomes in five Burkitt lymphoma cell lines.  J.          Natl. Cancer Inst. 38: 209-222, 1967. PubMed: 6019732
26256: . .  In Vitro 4: 165, 1969.
32796: Bloom TJ, Beavo JA. Identification          and tissue-specific expression of PDE7 phosphodiesterase          splice variants.  Proc. Natl. Acad. Sci. USA 93: 14188-14192,          1996. PubMed: 8943082