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平臺編號：bio-69428
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國際編號：CRL-1423?
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細(xì)胞信息： G-292, clone A141B1
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
G-292, clone A141B1
生長狀態(tài)：貼壁生長
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
年限：9 years
ATCC Number：CRL-1423?
相關(guān)疾病：骨肉瘤
運(yùn)輸方式：凍存運(yùn)輸
器官來源：骨
細(xì)胞形態(tài)：成纖維樣
規(guī)格：60 次×3 Designations: G-292, clone A141B1
Depositors: ?PT Peebles
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF)
Isoenzymes: G6PD, B
Age: 9 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22209: . . Pediatr. Res. 12: 485, 1978.
23015: Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. PubMed: 7860643
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
年限：9 years
ATCC Number：CRL-1423?
相關(guān)疾病：骨肉瘤
運(yùn)輸方式：凍存運(yùn)輸
器官來源：骨
細(xì)胞形態(tài)：成纖維樣
規(guī)格：60 次×3 Designations: G-292, clone A141B1
Depositors: ?PT Peebles
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:epidermal growth factor (EGF)
Isoenzymes: G6PD, B
Age: 9 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after seeding from a thawed ampule, then 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22209: . . Pediatr. Res. 12: 485, 1978.
23015: Zhang W, et al. EGF-mediated phosphorylation of extracellular signal-regulated kinases in osteoblastic cells. J. Cell. Physiol. 162: 348-358, 1995. PubMed: 7860643
