ATCC Number：CRL-1598?
相關疾?。簷M紋肌肉瘤
組織來源：muscle
生長狀態(tài)：貼壁生長
年限：15 years
是否是腫瘤細胞：1
物種來源：人
運輸方式：凍存運輸
數量：大量
細胞形態(tài)：多邊形
規(guī)格：60 次×3 Designations: A-673
Depositors: ?SA Aaronson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:polygonal
Source: Tissue: muscle
Disease: rhabdomyosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18
Age: 15 years
Gender: female
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse monolayer with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
