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器官來源：皮膚
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
年限：54 years
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CRL-1872?
相關(guān)疾病：惡性黑色素瘤
規(guī)格：60 次 Designations: A375.S2
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:

Source: Organ: skin
Disease: malignant melanoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
Age: 54 years
Gender: female
Comments:This line was cloned by limiting dilution from the A375 melanoma cell line (see ATCC CRL-1619 ).
These cells will eventually die and lyse when cultured in medium containing interleukin-1 (interleukin 1, IL-1) and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.
Sensitivity to IL-1 is maximal when the cells are derived from subconfluent cultures in logarithmic growth.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1619
References: 1773: Onozaki K, et al. Human interleukin-1 is a cytocidal factor for several tumor cell lines. J. Immunol. 135: 3962-3968, 1985. PubMed: 2415593
1774: Lachman LB, et al. Natural and recombinant human interleukin-1beta is cytotoxic for human melanoma cells. J. Immunol. 136: 3098-3102, 1986. PubMed: 3485680
1775: Nakai S, et al. A simple, sensitive bioassay for the detection of interleukin-1 using human melanoma A375 cell line. Biochem. Biophys. Res. Commun. 154: 1189-1196, 1988. PubMed: 3261584
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758