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平臺編號：bio-69449
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國際編號：CRL-1595?
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細胞信息： C-4 II
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
C-4 II
數(shù)量：大量
ATCC Number：CRL-1595?
相關疾?。耗[瘤
器官來源：宮頸
細胞形態(tài)：上皮樣
是否是腫瘤細胞：1
物種來源：人
年限：41 years
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸 Designations: C-4 II
Depositors: ?N Auersperg
Biosafety Level:2 [Cells contain human papilloma virus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cervix
Disease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 9,11
D7S820: 10,11
THO1: 9,9.3
TPOX: 10,11
vWA: 14
Isoenzymes: G6PD, B
Age: 41 years
Gender: female
Ethnicity: Caucasian
Comments:This is one of two lines developed from this patient (see ATCC CRL 1594).
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 22514: Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
26098: Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380
26099: Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382
26100: Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250
26101: Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980. PubMed: 6768754
ATCC Number：CRL-1595?
相關疾?。耗[瘤
器官來源：宮頸
細胞形態(tài)：上皮樣
是否是腫瘤細胞：1
物種來源：人
年限：41 years
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸 Designations: C-4 II
Depositors: ?N Auersperg
Biosafety Level:2 [Cells contain human papilloma virus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cervix
Disease: carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 9,11
D7S820: 10,11
THO1: 9,9.3
TPOX: 10,11
vWA: 14
Isoenzymes: G6PD, B
Age: 41 years
Gender: female
Ethnicity: Caucasian
Comments:This is one of two lines developed from this patient (see ATCC CRL 1594).
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 22514: Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
26098: Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380
26099: Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382
26100: Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250
26101: Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980. PubMed: 6768754
