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平臺編號：bio-69457
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國際編號：CRL-2592?
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細胞信息： A431NS
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
A431NS
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
數(shù)量：大量
細胞形態(tài)：上皮樣
器官來源：皮膚
組織來源：epidermis
ATCC Number：CRL-2592?
相關疾?。荷掀ぜ毎?br /> 是否是腫瘤細胞：1
物種來源：人
年限：85 yrs
規(guī)格：100次 Designations: A431NS
Depositors: ?K Hirabayashi
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial
Source: Organ: skin
Tissue: epidermis
Disease: epidermoid carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 9,13
D16S539: 12,14
D5S818: 12,13
D7S820: 10
THO1: 9
TPOX: 11
vWA: 15,17
Age: 85 yrs
Gender: female
Comments:A431NS was derived from the A431 cell line (ATCC CRL-1555 ) in 1997 by repeated subculturing to select cells that detached from the substrate easily.
The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis. [51526 ]
LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.
While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium and rinse with PBS. Remove the PBS completely and add fresh 0.25% trypsin, 0.03% EDTA solution. Allow the flask to sit at room temperature (or 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperture
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1555
References: 51526: Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480
運輸方式：凍存運輸
數(shù)量：大量
細胞形態(tài)：上皮樣
器官來源：皮膚
組織來源：epidermis
ATCC Number：CRL-2592?
相關疾?。荷掀ぜ毎?br /> 是否是腫瘤細胞：1
物種來源：人
年限：85 yrs
規(guī)格：100次 Designations: A431NS
Depositors: ?K Hirabayashi
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial
Source: Organ: skin
Tissue: epidermis
Disease: epidermoid carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 9,13
D16S539: 12,14
D5S818: 12,13
D7S820: 10
THO1: 9
TPOX: 11
vWA: 15,17
Age: 85 yrs
Gender: female
Comments:A431NS was derived from the A431 cell line (ATCC CRL-1555 ) in 1997 by repeated subculturing to select cells that detached from the substrate easily.
The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis. [51526 ]
LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.
While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium and rinse with PBS. Remove the PBS completely and add fresh 0.25% trypsin, 0.03% EDTA solution. Allow the flask to sit at room temperature (or 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperture
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-1555
References: 51526: Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480
