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平臺(tái)編號(hào)：bio-69474
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國(guó)際編號(hào)：CRL-7637?
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細(xì)胞信息： Hs 895.T
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Hs 895.T
細(xì)胞形態(tài)：成纖維樣
是否是腫瘤細(xì)胞：1
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
年限：48 years
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來源：皮膚
ATCC Number：CRL-7637?
相關(guān)疾病：黑色素瘤 Designations: Hs 895.T
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,14
D5S818: 11,12
D7S820: 10,12
THO1: 6,7
TPOX: 8,11
vWA: 18
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments:Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636 ), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2002
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020
derived from same individual: ATCC CRL-7636, Hs 895
是否是腫瘤細(xì)胞：1
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
年限：48 years
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來源：皮膚
ATCC Number：CRL-7637?
相關(guān)疾病：黑色素瘤 Designations: Hs 895.T
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,14
D5S818: 11,12
D7S820: 10,12
THO1: 6,7
TPOX: 8,11
vWA: 18
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments:Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636 ), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2002
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020
derived from same individual: ATCC CRL-7636, Hs 895
