生長狀態(tài)：貼壁生長
器官來源：皮膚
運輸方式：凍存運輸
數(shù)量：大量
ATCC Number：HTB-140?
相關(guān)疾病：黑色素瘤
是否是腫瘤細胞：1
物種來源：人
細胞形態(tài)：其他
年限：56 years
規(guī)格：0.1ml Designations: Hs 294T
Depositors: ?RB Owens
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixed stellate and polygonal
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:nerve growth factor (NGF); interferon
Tumorigenic:Yes
Cytogenetic Analysis:(P26) heteroploid with modal number = 59; Y chromosomes present
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
Me-2, 2
PGM1, 1-2
PGM3, 1
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments:The proliferative rate of the cell line is enhanced by addition of retinoic acid.
Responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon.
Results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma.
ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 1125: Czarniecki CW, et al. Synergistic antiviral and anti-proliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. J. Virol. 49: 490-496, 1984. PubMed: 6319748
1126: Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934
2155: Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204
22590: Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522
22658: Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563
26329: Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741
