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平臺(tái)編號(hào)：bio-69565
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國(guó)際編號(hào)：CRL-2105?
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細(xì)胞信息： HH
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
HH
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
年限：61 years adult
ATCC Number：CRL-2105?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾病：其他疾病
細(xì)胞形態(tài)：淋巴樣
器官來(lái)源：外周血
規(guī)格：0.1mg Designations: HH
Depositors: ?G Starkebaum
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: cutaneous T cell lymphoma
Cell Type: lymphocyte T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2) beta chain, expressed
Reverse Transcript:N
Antigen Expression:CD2; Homo sapiens, expressed
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD8; Homo sapiens; negative
CD25; Homo sapiens; negative
CD30; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12
D5S818: 11,13
D7S820: 10
THO1: 9
TPOX: 8,11
vWA: 15,16
Age: 61 years adult
Gender: male
Ethnicity: Caucasian
HeLa Markers: N
Comments:The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis. Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.
A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22863: Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
年限：61 years adult
ATCC Number：CRL-2105?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾病：其他疾病
細(xì)胞形態(tài)：淋巴樣
器官來(lái)源：外周血
規(guī)格：0.1mg Designations: HH
Depositors: ?G Starkebaum
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: cutaneous T cell lymphoma
Cell Type: lymphocyte T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2) beta chain, expressed
Reverse Transcript:N
Antigen Expression:CD2; Homo sapiens, expressed
CD3; Homo sapiens, expressed
CD4; Homo sapiens, expressed
CD8; Homo sapiens; negative
CD25; Homo sapiens; negative
CD30; Homo sapiens, expressed
HLA-DR; Homo sapiens, expressed
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 12
D5S818: 11,13
D7S820: 10
THO1: 9
TPOX: 8,11
vWA: 15,16
Age: 61 years adult
Gender: male
Ethnicity: Caucasian
HeLa Markers: N
Comments:The line was isolated from a patient who was negative for anti human T lymphotropic virus type I (HTLV-I) antibody, and no HTLV-I sequences were detected in the cultured cells by Southern analysis. Although HH cells lack the alpha chain of the IL-2 receptor (IL-2R alpha), they do possess a functional p70 beta chain (IL-2R beta) of the IL-2 receptor complex.
A culture submitted to the ATCC in June of 1992 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22863: Starkebaum G, et al. Establishment of an IL-2 independent, human T-cell line possessing only the p70 IL-2 receptor. Int. J. Cancer 49: 246-253, 1991. PubMed: 1879969
