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生長狀態(tài)：混合型生長
運輸方式：凍存運輸
數(shù)量：大量
器官來源：肺
細(xì)胞形態(tài)：上皮樣
ATCC Number：HTB-184?
相關(guān)疾?。浩渌膊?br /> 年限：56 years
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：0.1ml Designations: NCI-H510A [H510A, NCI-H510]
Depositors: ?AF Gazdar, JD Minna
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: lung
Disease: carcinoma; small cell lung cancer; extrapulmonary origin
Derived from metastatic site: adrenal gland
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an adult male patient.
The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
The line does not exhibit any gross structural DNA abnormalities.
The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 13
D5S818: 9,12
THO1: 7,9.3
TPOX: 8
vWA: 15
Cytogenetic Analysis:hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes were common to all cells. These included t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. Neither DM nor HSR were detected; structurally normal N1, N2, N13 and N21 were absent. Generally, there were 3 copies of both F group chromosomes; the X chromosomes were paired, and structurally normal Y chromosome was not found.
Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments:The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from an adrenal metastasis in an adult male patient.
The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue.
The line does not exhibit any gross structural DNA abnormalities.
The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: This line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium, collecting the cells by centrifugation, resuspending in fresh medium and dispensing into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
References: 1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257