年限：41 years
數(shù)量：大量
ATCC Number：CRL-2138?
相關(guān)疾?。浩渌膊?br />
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：100 ul Designations: VA-ES-BJ
Depositors: ?C Helson
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Disease: epithelioid carcinoma
Derived from metastatic site: bone marrow
Cellular Products:granulocyte macrophage colony stimulating factor (GM-CSF)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Oncogene:p53 +
Cytogenetic Analysis:near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent.
Age: 41 years
Gender: male
Ethnicity: Caucasian
Comments:VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma.
The cells are sensitive to hemin.
The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22334: . . Int. J. Oncol. 7: 51-56, 1995.
