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ATCC Number：PCS-100-020
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
細(xì)胞形態(tài)：其他
年限：Lot-specific
數(shù)量：大量
規(guī)格：0.1ml Name: Primary Coronary Artery Endothelial Cells; Normal, Human
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Coronary artery
Growth Properties: Adherent
Age: Lot-specific
Morphology: Cobblestone appearance
Volume per Vial: 1 ml
Ethnicity: Lot-specific
Gender: Lot-specific
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Primary Coronary Artery Smooth Muscle Cells; Normal, Human (ATCC PCS-100-021 )
Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040 )
Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
Recomended Culture Conditions:See Product Information Sheet Comments:The cells are cryopreserved in the third passage to ensure the highest purity, coupled with high viability and plating efficiency.
A complete solution to propagate coronary artery endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified recombinant human (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Angiogenesis, arteriolosclerosis, hypertension, oncology, drug screening, inflammation, vasodilator function.
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV-1 and HIV 2: Negative