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運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
年限：Neonatal
ATCC Number：PCS-201-011
細(xì)胞形態(tài)：其他
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：0.1ml Name: Dermal Fibroblasts; Normal, Human, Neonatal, Mitomycin C Treated
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Foreskin
Growth Properties: Adherent
Age: Neonatal
Morphology: Fibroblast-like; normal for serum-free growth
Volume per Vial: 1 ml
Gender: Male
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Dermal Fibroblasts; Normal, Human, Adult (ATCC PCS-201-012 )
Dermal Fibroblasts; Normal, Human, Neonatal (ATCC PCS-201-010 )
Fibroblast Basal Medium (ATCC PCS-201-030 )
Fibroblast Growth Kit, Serum-free (ATCC PCS-201-040 )
Fibroblast Growth Kit, Low serum (ATCC PCS-201-041 )
Dulbecco's Phosphate Buffered Saline, (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
0.1% Gelatin Solution (ATCC PCS-999-027 )
Recomended Culture Conditions:See Product Information Sheet Comments:Primary neonatal fibroblasts have been treated with mitomycin C at passage #1 and will not replicate. Once the feeder cells have attached, the culture medium should be changed to accommodate the cells to be supported.
Applications:The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. Suitable for use with other cell types that require a feeder layer for optimal attachment and proliferation.
Viability:≥ 50%
Population Doubling Capacity:No growth/division over 4 weeks
Sterility Tests: Bacteria and Yeasts: Negative.
Mycoplasma: Negative
Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV: Negative