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生長狀態(tài)：貼壁生長
數量：大量
年限：Neonatal
運輸方式：凍存運輸
ATCC Number：PCS-100-013
細胞形態(tài)：其他
是否是腫瘤細胞：0
物種來源：人
規(guī)格：0.1ml Name: Primary Umbilical Vein Endothelial Cells; Normal, Human, Pooled
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Umbilical vein endothelium
Growth Properties: Adherent
Age: Neonatal
Morphology: Cobblestone appearance
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Vascular Cell Basal Medium (ATCC PCS-100-030 )
Endothelial Cell Growth Kit/BBE (ATCC PCS-100-040 )
Endothelial Cell Growth Kit/VEGF (ATCC PCS-100-041 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-000-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )
Recomended Culture Conditions:See Product Information Sheet Comments:The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. ATCC primary pooled HUVECs are derived from 10 individual donors, minimizing the lot-to-lot variability associated with cells derived from single donors. Large lot sizes assure consistency for large-scale studies.
A complete solution to propagate pooled HUVECs in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired.
Applications:Drug screening, angiogenesis, tubule formation, immune response, arteriolosclerosis, hypertension, vascular wound healing, inflammation, tissue remodeling, vascular differentiation, oxidative stress, apoptosis, stent development
Viability:≥ 50%
Population Doubling Capacity:≥ 15 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative

Testing: Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
HIV-1 and HIV-2: Negative