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數(shù)量：大量
運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：人
ATCC Number：PCS-200-013
細胞形態(tài)：其他
規(guī)格：0.1ml Name: Primary Epidermal Melanoyctes; Normal, Human, Adult
Biosafety Level:1
Shipped: frozen
Organism: Homo sapiens (human)
Source: Epidermis (basal layer)
Foreskin, lightly pigmented
Growth Properties: Adherent
Morphology: Stellar, multipolar or dendritic apperance; needle-like
Volume per Vial: 1 ml
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Products:Dermal Cell Basal Medium (ATCC PCS-200-030 )
Adult Melanocyte Growth Kit (ATCC PCS-200-042 )
Dulbecco's Phosphate Buffered Saline (D-PBS) (ATCC 30-2200 )
Trypsin-EDTA for Primary Cells (ATCC PCS-999-003 )
Trypsin Neutralizing Solution (ATCC PCS-999-004 )
Gentamicin-Amphotericin B Solution (ATCC PCS-999-025 )
Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002 )
Phenol Red (ATCC PCS-999-001 )

Recomended Culture Conditions:See Product Information Sheet Comments:ATCC ^? Normal Human Primary Epidermal Melanocytes from from adult tissue, when grown in Dermal Cell Basal Media supplemented with Adult Melanocyte Growth Kit components, provide an ideal cell system to propagate adult melanocytes in low serum (less than 1.0% FBS) conditions in the absence of cholera toxin and phorbol 12-myristate 13-acetate (PMA). The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.
Applications:Melanoma, response to UV radiation, psoriasis and other skin diseases, skin trauma (e.g., wound repair, scars, burns), cosmetic research (e.g., skin lightening compounds, skin protecting compounds)
Viability:≥ 50%
Population Doubling Capacity:≥ 10 in complete growth medium
Sterility Tests: Bacteria and Yeast: Negative
Mycoplasma: Negative
Testing: Hepatitis B: Negative
Hepatitis C: Negative
HIV-1 and HIV-2 Testing: Negative