產(chǎn)品信息
名稱(chēng) SU-DHL-1 (人間變性大細(xì)胞淋巴瘤細(xì)胞) (STR鑒定正確)
別稱(chēng) SU-DHL1; SUDHL1; SUDHL-1; SuDHL-1; SuDHL 1; Stanford University-Diffuse Histiocytic Lymphoma-1
種屬 人源
生長(zhǎng)特性 懸浮細(xì)胞
細(xì)胞形態(tài) 淋巴母細(xì)胞樣
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)方案A(默認(rèn)) 生長(zhǎng)培養(yǎng)基:RPMI-1640+10% FBS+1% P/S培養(yǎng)條件:氣相:空氣,95%;CO2,5%, 溫度:37℃
推薦傳代比例 1:2-1:6
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 SU-DHL-1 is a histiocytic cell that was isolated from the lymph node of a White, 10-year-old, male patient with large cell lymphoma. The cells are surface Ig negative (sIG-). The cells are non-specific esterase, acid phosphatase and oil red O positive. The cells are periodic acid Schiff negative. These cells phagocytose Candida albicans and latex particles. The cells are reported to be very weakly E - rosette positive. ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.
年齡(性別) 男性;10歲
組織來(lái)源 淋巴結(jié)
細(xì)胞類(lèi)型 腫瘤細(xì)胞
腫瘤類(lèi)型 淋巴瘤細(xì)胞
生物安全等級(jí) BSL-1
倍增時(shí)間 ~40-50 hours (DSMZ=ACC-356)
受體表達(dá)情況 CFS-1, expressed; (IL-1-R; IL-2-Rα; IL-6-R; TNFα-R; & c-fms), expressed; Surface Receptors: (Fc; IgMEAC; IgGEA; & E), not expressed
抗原表達(dá)情況 Monocyte Marker: CD163+ Lymphoid Marker: CD45- Progenitor Markers: CD10-, CD34- Activation Markers: CD30+, CD25+, CD70+, CD71+, CD80-, HLA-DR+, CD45- T-Cell Markers: CD2-, CD3-, CD4-, CD5+, CD7-, CD8- B-Cell Markers: CD19-, CD20-, CD21-, CD22- Myelom
基因表達(dá)情況 fusion gene NPM-ALK (p80)+; anaplastic lymphoma kinase (ALK)+; Ig not expressed
保藏機(jī)構(gòu) ATCC; CRL-2955