產(chǎn)品信息
名稱 sNF96.2 (人雪旺細胞 (神經(jīng)纖維瘤病Ⅰ型)) (STR鑒定正確)
別稱 SNF96.2; sNF96-2
種屬 人
生長特性 貼壁細胞
細胞形態(tài) 雪旺細胞樣
凍存條件 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)方案A(默認) 生長培養(yǎng)基:DMEM+10%FBS+1%P/S培養(yǎng)條件:氣相:空氣,95%;CO2,5%, 溫度:37℃
推薦傳代比例 1:2-1:3
推薦換液頻率 2-3次/周
參考資料(來源文獻)
背景描述 sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75.
年齡(性別) 男性;27歲
細胞類型 腫瘤細胞
腫瘤類型 雪旺細胞樣
生物安全等級 BSL-1
倍增時間 ~33 hours (ATCC=CRL-2884)
保藏機構(gòu) ATCC; CRL-2884