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　　細(xì)胞名稱(chēng)：人胃癌細(xì)胞SNU-5

產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2

細(xì)胞數(shù)量：1x10^6；1x10^6

保存溫度：37℃；-198℃

運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸

安全等級(jí)：1

用途限制：僅供科研3類(lèi)

培養(yǎng)體系：DMEM高糖（不含丙酮酸鈉）+10%FBS+1%三抗

培養(yǎng)溫度：37℃

二氧化碳濃度：5%

簡(jiǎn)介：人胃癌細(xì)胞SNU-5取自33歲女性供體，貼壁培養(yǎng)。

注釋?zhuān)篜art of:Cancer Cell Line Encyclopedia(CCLE)project.

Part of:COSMIC cell lines project.

Part of:MD Anderson Cell Lines Project.

Part of:MET genetic alteration cell panel(ATCC TCP-1036).

Part of:Seoul National University(SNU)cell line collection.

Population:Korean.

Doubling time:34 hours(PubMed=2158397).

Microsatellite instability:Stable(MSS)(Sanger).

Omics:Deep exome analysis.

Omics:Deep RNAseq analysis.

Omics:DNA methylation analysis.

Omics:Protein expression by reverse-phase protein arrays.

Omics:SNP array analysis.

Omics:Transcriptome analysis.

突變信息

TP53 p.Gly262_Ser269delGlyAsnLeuLeuGlyArgAsnSer(c.784_807del24)(PubMed=1676761).

STR信息

Amelogenin X

CSF1PO 12

D2S1338 17

D3S1358 16

D5S818 10

D7S820 8,12

D8S1179 14,15

D13S317 8(KCLB)

8,9(ATCC;Cosmic-CLP;PubMed=25877200)

D16S539 13

D18S51 13

D19S433 13.2,15

D21S11 29

FGA 21

Penta D 9,13

Penta E 18,19

TH01 9

TPOX 11

vWA 15,16

參考文獻(xiàn)

PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747

Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res.79:1263-1273(2019)

PubMed=31068700;DOI=10.1038/s41586-019-1186-3

Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

細(xì)胞操作說(shuō)明書(shū)下載：細(xì)胞操作說(shuō)明書(shū)