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平臺(tái)編號(hào)：bio-74185
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國際編號(hào)：ATCC HTB-32
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細(xì)胞信息： Homo sapiens, human
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
HT-3*
HT-3
(ATCC? HTB-32?)
Organism: Homo sapiens, human / Cell
Type: Retinoblastoma /
Tissue: cervix; Derived from metastic site: lymph node, carcinoma /
Disease: Retinoblastoma, Papilloma
Organism Homo sapiens, human
Tissue cervix; Derived from metastic site: lymph node, carcinoma
Cell Type Retinoblastoma
Product Format frozen
Morphology epithelial Culture Properties adherent
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Retinoblastoma, Papilloma
Age 58 years
Gender female
Ethnicity Caucasia
Storage Conditions liquid nitrogen vapor phase
Karyotype (P147) hypotriploid to hypertriploid (+A, +B, +C, +D, +F, -E). Modal number = 65 to 66 with abnormalities including dicentrics, acrocentric fragments, minutes, translocations, rings, secondary constrictions, breaks, pulverizations and large subtelocentric and large submetacentric markers.
Clinical Data 58 years Caucasian female
Antigen Expression Blood Type A; Rh+
Oncogene p53 +; pRB +
Tumorigenic Yes
Effects Yes, in nude mice; forms poorly differentiated epidermoid carcinoma (grade III); also forms tumors in steroid treated hamsters
Comments Ultrastructural features include many microvilli, prominent nucleoli, sparse RER, well developed Golgi.
The retinoblastoma protein (pRB) is present but abnormal in size. P53 expression is elevated and there is a point mutation at codon 245 resulting in a Gly -> Val substitution The line is negative for human papillomavirus DNA and RNA. No virus particles were observed.
A mycoplasma contamination was detected and eliminated in 1965.
Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, rinse with fresh 0.25% trypsin-0.53 mM EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37℃) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37℃
STR Profile Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 12,13 D5S818: 10,13 D7S820: 8,10 THO1: 6,7 TPOX: 8 vWA: 15,18
Isoenzymes AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1
