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hMR(1-516) 拉丁名

(ATCC? 80020?) 統(tǒng)一編號(hào)

Designations hMR(1-516)

GenBank Number M80582

Species Homo sapiens, human

Depositors MV Govindan

Vector Construct size (kb): 6.619999885559082 DESCRIPTION OF VECTOR COMPONENT:

Name of vector: p

Bluescript SK- Intact vector size: 2.960

Type of vector: phagemid

Vector end: EcoRI

Vector end: EcoRI

Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI

Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI)

KpnI Construction: pUC19

Host range: Escherichia coli

Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ', <- primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <-

Cross references: Insert DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: mLR

Genomic copy number: unique Gene name: mineralocorticoid receptor (aldosterone receptor) Contains complete coding sequence?: U Chromosome: 4; Localization: 4 q31; 4, cR

Tissue: lymphocyte

Type of DNA: genomic

Insert end: EcoRI

Insert end: EcoRI

Insert size (kb): 3.677

Cross references: DNA Seq. Acc.: M80582 Nucleotides 1-3677 of the insert correspond to nucleotides 1-3677 of M80582. hMR(1-516) (ATCC? 80020?)

Insert lengths(kb): 3.677000045776367

Tissue: lymphocyte Gene product: mineralocorticoid receptor (aldosterone receptor) [MLR] Insert Size (kb) 3.677

Media ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level 生物安全等級(jí) 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried

Comments 注釋 Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 3.4; EcoRI-- 4.0, 3.0; HindIII--4.3, 2.7; KpnI--4.4, 2.5; XbaI--4.5, 2.3.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--3.17; BglII--1.46; HindIII--1.0; KpnI--1.34; NcoI--0.67, 2.43; PstI--2.96; PvuII--2.54; SacI--2.63; XbaI--2.16.

Contains regulatory elements and an open reading frame for aa 1-516 (nt 2124-3677), terminating at the EcoRI site.

References 參考文獻(xiàn) Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951