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平臺編號：bio-79778
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國際編號：ATCC CRL-6323
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細(xì)胞信息： Mus musculus, mouse
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
B16-F1
B16-F1 英文
(ATCC? CRL-6323?) 統(tǒng)一編號
Organism 品系 : Mus musculus, mouse /
Tissue 組織 : skin /
Disease: melanoma
Product Format 提供形式 frozen
Morphology mixture of spindle-shaped and epithelial-like cells
Culture Properties adherent
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease melanoma
Strain C57BL/6J
Applications 用途 This cell line is a suitable transfection host. Storage Conditions liquid nitrogen vapor phase T
umorigenic Yes
Effects Yes, in syngeneic mice
Complete Growth Medium 完全培養(yǎng)基 The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37℃) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation 冷凍方法 Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions 培養(yǎng)條件 Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37℃
