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平臺編號：bio-81983
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國際編號：ATCC 95487
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細(xì)胞信息： D15
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規(guī)格：frozen
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用途：ATCC原裝進(jìn)口
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
D15
D15
(ATCC? 95487?) 統(tǒng)一編號
Designations 別名 D15
Gen Bank Number L10820
Species Homo sapiens, human
Vector Construct size (kb): 4.349999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK- Intact vector
size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI
Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ',promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, <- promoter: lac, <- primer site: M13 reverse, <- replicon: pMB1 marker(s): ampR, <-
Cross references: Insert DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens Gene symbol: FPR1 Gene name: formyl peptide receptor 1 Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.4 Tissue: HL-60 cell line Disease: promyelocytic leukemia
Type of DNA: cDNA
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Insert size (kb): 1.35
Cross references: DNA Seq. Acc.: L10820
Nucleotides 1-1350 of the insert correspond to nucleotides 525-552, 5370-6599 of L10820.
Insert lengths(kb): 1.350000023841858
Tissue: HL-60 cell line promyelocytic leukemia
Disease: promyelocytic leukemia
Gene product: formyl peptide receptor 1 [FPR1]
Insert Size (kb) 1.350
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1.
The insert includes the following restriction sites (approximate kb from the 5' end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18.
Lacks exon 2 in the 5' untranslated region that is present in some alternatively spliced transcripts.
Begins within exon 1 and contains the complete exon 3. References Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916
Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023
Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134
ATCC95487
