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BEND 拉丁名

(ATCC? CRL-2398?) 統(tǒng)一編號(hào)

Organism Bos taurus, cow 

Tissue uterus, endometrium 

Product Format 提供形式 frozen 

Morphology epithelial 

Culture Properties adherent 

Biosafety Level 生物安全等級(jí) 2 [Cells contain bovine viral diarrhea virus (BVDV)] 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Disease normal 

Age adult; day 14 of estrous cycle 

Gender female 

Storage Conditions 保藏條件 liquid nitrogen vapor phase

Derivation The BEND cell line was derived from the uterine endometrium of a normal female cow on day 14 of the estrous cycle in 1997 in Laramie Wyoming, United States. 

Cellular Products ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to interferon 

Comments 注釋 The cells produce the pregnancy associated protein called bovine ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to the cytokine interferon-tau. 

BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6. 

Tests for bovine viral diarrhea virus (BVDV) at the ATCC have indicated that BEND cells are positive for BVDV by immunofluorescence.

Complete Growth Medium 1:1 mixture of Ham's F12 and Eagle's Minimal Essential medium with Earle's BSS (D-valine modification) with 1.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.034 g/L D-valine, 10% heat-inactivated fetal bovine serum and 10% heat-inactivated horse serum Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

Remove and discard culture medium. 

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. 

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). 

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. 

Incubate cultures at 37°C. 

Subcultivation Ratio: 1:4 to 1:6 

Medium Renewal: Every 2 to 3 days 

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. 

Cryopreservation Complete growth medium described above supplemented with 5% (v/v) DMSO.  

Cell culture tested DMSO is available as ATCC Catalog No. 4-X. 

Culture Conditions 培養(yǎng)條件 

Temperature 培養(yǎng)溫度 : 37°C 

Atmosphere 需氧情況 : Air, 95%; Carbon dioxide (CO2), 5%

Name of Depositor 寄存人 TR Hansen, KJ Austin, GA Johnson 

Deposited As Bos taurus 

Passage History BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6. 

Year of Origin 1997 

References 參考文獻(xiàn) Staggs KL, et al. Complex induction of bovine uterine proteins by interferon-tau. Biol. Reprod. 59: 293-297, 1998. PubMed: 9687298 

Hansen TR, et al. Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 138: 5079-5082, 1997. PubMed: 9348245 

Austin KJ, et al. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 140: 542-545, 1999. PubMed: 9886868 

Johnson GA, et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine 10: 243-252, 1999. PubMed: 10484288 

Perry DJ, et al. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol. Endocrinol. 13: 1197-1206, 1999. PubMed: 10406469 

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. 

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. 

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) 

Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. 

HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.