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平臺(tái)編號(hào)：bio-109371
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質(zhì)粒信息： 宿主：BL21
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規(guī)格：2ml甘油菌 20ul質(zhì)粒
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用途：哺乳細(xì)胞雙雜交載體質(zhì)粒
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pACT
| 啟動(dòng)子 | CMV |
| 復(fù)制子 | pUC |
| 終止子 | SV40 poly(A) signal |
| 質(zhì)粒分類 | 哺乳細(xì)胞，哺乳細(xì)胞雙雜交載體 |
| 質(zhì)粒大小 | 5566bp |
| 原核抗性 | Amp |
| 篩選標(biāo)記 | G418 |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 表達(dá)宿主 | 哺乳細(xì)胞 |
| 誘導(dǎo)方式 | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
| 載體宿主 | 哺乳細(xì)胞 |
| 載體用途 | 雜交 |
| 原核抗性 | Amp |
| 真核抗性 | Neo/G418 |
| 5'測(cè)序引物 | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'測(cè)序引物 | PIRES.R：CACTGCATTCTAGTTGTGGT |
| 質(zhì)粒簡(jiǎn)介 | The pACT Vector is a high-copy plasmid in which the human cytomegalovirus (CMV) immediate early promoter drives expression of the herpes virus VP16activation domain (amino acids 411–456). A chimeric intron is located 5´ of the gene segment, and a multiple cloning region is located 3´ of the gene segment for insertion of cDNA clones of interest . The presence of this chimeric intron, in context with the CMV promoter and polyadenylation signal, can result in increased protein expression of cDNA genes linked to these elements. The stop codons and SV40 late polyadenylation region, demonstrated to be efficient for mRNA production , are at the 3´ end of the fusion gene. The fusion gene region is flanked by T7 and T3 RNA polymerase promoters for the synthesis of sense and antisense RNA products. The T7 promoter allows the construct to be translated using the TNT T7 Quick Coupled Transcription/Translation System (Cat.). Also located on this vector is the neomycin phosphotransferase gene (from Tn5) driven by the SV40 early promoter and followed by a synthetic polyadenylation cassette. This gene confers resistance to the neomycin analog, G418 . The plasmid backbone contains an f1 origin of replication for the production of single-stranded DNA (ssDNA) and the β-lactamase (Ampr) gene for selection of the vector DNA in E. coli. |
