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平臺編號：bio-109376
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質粒信息： 宿主：DH5α，BL21
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規(guī)格：2ml甘油菌 20ul質粒
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用途：酵母系列，酵母單雜交載體，配套pGADT7，低拷貝載體
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
pAbAi
| 啟動子 | URA3 |
| 復制子 | pUC |
| 質粒分類 | 酵母系列質粒；酵母雜交質粒；單雜交類質粒 |
| 質粒大小 | 4894bp |
| 原核抗性 | Amp |
| 篩選標記 | URA3 |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 30℃，YPDA，有氧 |
| 表達宿主 | Y1Hgold等酵母菌 |
| 載體宿主 | 酵母菌 |
| 載體用途 | 雜交 |
| 原核抗性 | Amp |
| 真核抗性 | URA3 |
| 5'測序引物 | pABAI-F（GTTCCTTATATGTAGCTTTCGACA） |
| 3'測序引物 | pABAI-R（CCATCTCGAAAAAGGGTTTGCC） |
| 備注 | 低拷貝質粒 |
| 質粒簡介 | pABAI質粒是一種單雜交酵母報告質粒，可以識別和分析蛋白質與DNA之間的相互作用，而不僅僅是證明蛋白之間的相互作用。這個質粒具有AbA抗性因而具有很強的篩選能力，可極大地降低背景水平。 pABAI is a yeast reporter vector that can be used in one-hybrid assays to identify and characterize DNA-binding proteins. The vector, which was specifically designed for use with the Matchmaker Gold Yeast One-Hybrid Library Screening System, contains a multiple cloning site upstream of the yeast iso-1-cytochrome C minimal promoter(not shown) and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A. A cis-acting element (i.e., a target sequence) can be inserted into the MCS and used as bait to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the target sequence. Positive protein-DNA (i.e.,one-hybrid) interactions drive the expression of AUR1-C. As a result, one-hybrid interactions can be detected by selecting for yeast that are resistant to AbA. pABAI cannot be propagated episomally in yeast; it can only be stablymaintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the ura3-52 locus of the yeast strain provided in theMatchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for selection of recombinant yeast. The vector also contains a Col E1 origin of replication and an ampicillin resistance gene (Ampr) for propagation and selection in E.coli. To use pAbAi in a one-hybrid assay, clone one or more copies of a cis-acting element into the MCS. To facilitate recombination of the vector with the host’s ura3-52 locus, linearize the vector with either BbsI or BstBI, then introduce the linearized vector into competent yeast cells using the protocols in the Matchmaker Gold Yeast One-Hybrid Library Screening System User Manual. Insertion of your target sequence may alter the basal expression of AUR1-C. Therefore, before starting a onehybrid analysis, basal levels of AUR1-C should be determined as described in the User Manual. • Suitable host strains: DH5α and other general purpose strains. • Selectable marker: plasmid confers resistance to ampicillin (100 µg/ml) to E. coli hosts. • Copy number: low |
