| 啟動(dòng)子 |
CMV |
| 復(fù)制子 |
pUC |
| 終止子 |
SV40 poly(A) signal,hGH poly(A) signal |
| 質(zhì)粒分類 |
哺乳細(xì)胞，蛋白過表達(dá)載體 |
| 質(zhì)粒大小 |
4717bp |
| 原核抗性 |
Amp |
| 篩選標(biāo)記 |
Neo |
| 克隆菌株 |
DH5a |
| 培養(yǎng)條件 |
37度 |
| 表達(dá)宿主 |
哺乳細(xì)胞 |
| 誘導(dǎo)方式 |
無須誘導(dǎo)，瞬時(shí)表達(dá) |
| 5'測(cè)序引物 |
CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'測(cè)序引物 |
hGH-PA-R:CCAGCTTGGTTCCCAATAGA |
| 載體宿主 |
哺乳細(xì)胞 |
| 載體用途 |
蛋白表達(dá) |
| 基因種屬 |
空載體 |
| 基因類型 |
ORF |
| 原核抗性 |
Amp |
| 質(zhì)粒簡(jiǎn)介 |
p3XFLAG-CMV-7.1 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish transient intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. |
