>中國(guó)微生物菌種>質(zhì)粒載體>pGADT7-T
| 培養(yǎng)條件 | LB,37℃,氨芐 |
| 啟動(dòng)子 | ADH1 |
| 復(fù)制子 | 2U |
| 質(zhì)粒分類 | 酵母系列,酵母雙雜交載體 |
| 質(zhì)粒大小 | 10.kb |
| 原核抗性 | Amp |
| 篩選標(biāo)記 | LEU2 |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 表達(dá)宿主 | 酵母細(xì)胞 |
| 5'測(cè)序引物 | T7:TAATACGACTCACTATAGGG |
| 3'測(cè)序引物 | 根據(jù)序列設(shè)計(jì)引物 |
| 載體宿主 | 酵母菌 |
| 載體用途 | 雜交 |
| 原核抗性 | Amp |
| 真核抗性 | LEU2 |
| 質(zhì)粒簡(jiǎn)介 | pGADT7-T encodes a fusion of the SV40 large T-antigen (a.a. 86–708) and the GAL4 AD (a.a. 768–881). The SV40 large T DNA (GenBank Locus SV4CG) was derived from a plasmid referenced in Li & Fields (1993) and was cloned into pGADT7 using the EcoR I and Xho I sites. pGADT7-T has not been sequenced. |