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平臺編號：bio-114783
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質(zhì)粒信息： pFast-Bac-HTC
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pFast-Bac-HTC
| 啟動(dòng)子 | PC,polyhedrin |
| 復(fù)制子 | pUC ori，F(xiàn)1 ori |
| 終止子 | SV40 poly(A) signal |
| 質(zhì)粒分類 | 廣宿主系列，昆蟲細(xì)胞載體 |
| 質(zhì)粒大小 | 4858bp |
| 質(zhì)粒標(biāo)簽 | N-His；N-TEV |
| 原核抗性 | Amp |
| 篩選標(biāo)記 | Gen |
| 克隆菌株 | DH5α |
| 培養(yǎng)條件 | 37℃，有氧 LB |
| 表達(dá)宿主 | 昆蟲細(xì)胞 |
| 誘導(dǎo)方式 | 無須誘導(dǎo)，瞬時(shí)表達(dá) |
| 5'測序引物 | pFastbac-F: TATTCCGGATTATTCATACC |
| 3'測序引物 | pFastBac-R: ACAAATGTGGTATGGCTGA |
| 質(zhì)粒簡介 | The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag. |
| 質(zhì)粒序列 |
LOCUS Exported 4858 bp ds-DNA circular SYN 07-SEP-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled 11 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4858) AUTHORS . TITLE Direct Submission JOURNAL Exported Wednesday, September 7, 2016 from SnapGene Viewer 3.1.4 FEATURES Location/Qualifiers source 1..4858 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 2..457 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 484..588 /gene="bla" /note="AmpR promoter" CDS 589..1449 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1620..2208 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element 2511..2735 /mobile_element_type="transposon:Tn7" /note="Tn7R" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2802..3335) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3524..3552) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" promoter 3904..3995 /gene="polh from Autographa californica" /note="polyhedrin promoter" /note="promoter for the baculovirus polyhedrin gene" CDS 4062..4079 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS 4101..4121 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /note="TEV site" /translation="ENLYFQG" polyA_signal 4348..4482 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" mobile_element 4511..4676 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat 481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct 2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga 2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag 2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt 2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga 2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac 2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg 2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg 2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca 2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact 3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc 3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta 3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct 3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg 3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg 3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca 3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa 3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa 3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca 3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac 3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc 3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg 3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt 3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt 3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa 3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg 4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgggg atccggaatt 4141 caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt 4201 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc 4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact agatctgcct 4501 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt 4561 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa 4621 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg 4681 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc 4741 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt 4801 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg |
