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平臺(tái)編號(hào)：bio-114877
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質(zhì)粒信息： pmCherry-Tubulin-C-18
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
pmCherry-Tubulin-C-18
| 別稱 | Plasmid#55148 |
| 復(fù)制子 | pUC |
| 質(zhì)粒分類 | 基因文庫質(zhì)粒 |
| 原核抗性 | Kan |
| 篩選標(biāo)記 | G418 |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 載體宿主 | 哺乳細(xì)胞 |
| 載體用途 | 蛋白表達(dá) |
| 基因種屬 | 人 |
| 基因類型 | ORF |
| 原核抗性 | Kan |
| 真核抗性 | Neo/G418 |
| 熒光蛋白 | 紅色 |
| 質(zhì)粒簡(jiǎn)介 | Homo sapiens tubulin alpha 1b (TUBA1B), mRNA |
| 質(zhì)粒序列 | LOCUS Exported 6072 bp ds-DNA circular SYN 25-FEB-2018
FEATURES Location/Qualifiers source 1..6072 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 613..1320 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" misc_feature 1376..2730 /label=Tubulin polyA_signal 2860..2981 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2988..3443) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3470..3574 /gene="bla" /label=AmpR promoter promoter 3576..3933 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3784..3919 /label=SV40 ori /note="SV40 origin of replication" CDS 3968..4762 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 4994..5041 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5370..5958 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc 661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt 781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc 841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc 901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc 1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac 1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc 1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc 1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg 1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag 1321 tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgtgcgt 1381 gagtgcatct ccatccacgt tggccaggct ggtgtccaga ttggcaatgc ctgctgggag 1441 ctctactgcc tggaacacgg catccagccc gatggccaga tgccaagtga caagaccatt 1501 gggggaggag atgactcctt caacaccttc ttcagtgaga cgggcgctgg caagcacgtg 1561 ccccgggctg tgtttgtaga cttggaaccc acagtcattg atgaagttcg cactggcacc 1621 taccgccagc tcttccaccc tgagcagctc atcacaggca aggaagatgc tgccaataac 1681 tatgcccgag ggcactacac cattggcaag gagatcattg accttgtgtt ggaccgaatt 1741 cgcaagctgg ctgaccagtg caccggtctt cagggcttct tggttttcca cagctttggt 1801 gggggaactg gttctgggtt cacctccctg ctcatggaac gtctctcagt tgattatggc 1861 aagaagtcca agctggagtt ctccatttac ccagcacccc aggtttccac agctgtagtt 1921 gagccctaca actccatcct caccacccac accaccctgg agcactctga ttgtgccttc 1981 atggtagaca atgaggccat ctatgacatc tgtcgtagaa acctcgatat cgagcgccca 2041 acctacacta accttaaccg ccttattagc cagattgtgt cctccatcac tgcttccctg 2101 agatttgatg gagccctgaa tgttgacctg acagaattcc agaccaacct ggtgccctac 2161 ccccgcatcc acttccctct ggccacatat gcccctgtca tctctgctga gaaagcctac 2221 catgaacagc tttctgtagc agagatcacc aatgcttgct ttgagccagc caaccagatg 2281 gtgaaatgtg accctcgcca tggtaaatac atggcttgct gcctgttgta ccgtggtgac 2341 gtggttccca aagatgtcaa tgctgccatt gccaccatca aaaccaagcg cagcatccag 2401 tttgtggatt ggtgccccac tggcttcaag gttggcatca actaccagcc tcccactgtg 2461 gtgcctggtg gagacctggc caaggtacag agagctgtgt gcatgctgag caacaccaca 2521 gccattgctg aggcctgggc tcgcctggac cacaagtttg acctgatgta tgccaagcgt 2581 gcctttgttc actggtacgt gggtgagggg atggaggaag gcgagttttc agaggcccgt 2641 gaagatatgg ctgcccttga gaaggattat gaggaggttg gtgtggattc tgttgaagga 2701 gagggtgagg aagaaggaga ggaatactaa ggatccaccg gatctagata actgatcata 2761 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 2821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 2881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 2941 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt 3001 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 3061 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 3121 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 3181 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 3241 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 3301 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 3361 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 3421 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 3541 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg 3601 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3661 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3721 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3781 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3841 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 3901 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc 3961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 4021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 4081 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 4141 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 4201 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 4261 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 4321 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 4381 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 4441 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat 4501 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 4561 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 4621 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 4681 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 4741 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg 4801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc 4861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag 4921 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga 4981 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt 5041 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat 5101 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa 5161 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca 5221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 5281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 5341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 5401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 5461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 5521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 5941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 6061 accgccatgc at |
