| 別稱 | pGL3.0-Control |
| 啟動(dòng)子 | SV40 |
| 復(fù)制子 | pUC |
| 終止子 | SV40 poly(A) signal |
| 質(zhì)粒分類 | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
| 質(zhì)粒大小 | 5256bp |
| 原核抗性 | Amp |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 表達(dá)宿主 | 哺乳細(xì)胞 |
| 誘導(dǎo)方式 | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
| 5'測(cè)序引物 | RVP3:CTAGCAAAATAGGCTGTCCC |
| 3'測(cè)序引物 | 根據(jù)序列設(shè)計(jì)引物 |
| 載體宿主 | 哺乳細(xì)胞 |
| 載體用途 | 啟動(dòng)子檢測(cè) |
| 基因種屬 | |
| 熒光蛋白 | fLuc |
| 原核抗性 | Amp |
| 基因類型 | Promoter |
| 質(zhì)粒簡(jiǎn)介 | The pGL3 Luciferase Reporter Vectors(a–c) provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL3 Luciferase Reporter Vectors is designed for increased expression, and contains a modified coding region for firefly (Photinus pyralis) luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, these Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation. |
質(zhì)粒序列LOCUS Exported File 5256 bp ds-DNA circular SYN 23-3-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pGL3-Control
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5256)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-3-23 from MLCC
FEATURES Location/Qualifiers
source 1..5256
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 48..244
/note="SV40 promoter"
/note="SV40 early promoter"
rep_origin 95..230
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 280..1932
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/EC_number="
"
/note="luciferase"
/note="enhanced?luc+ version of the luciferase gene"
/protein_id="
"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1973..2094
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2759..3347)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3518..4378)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4379..4483)
/gene="bla"
/note="AmpR promoter"
rep_origin 4510..4965
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 5096..5144
/note="synthetic polyadenylation signal"
misc_feature 5158..5249
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human?alpha-2 globin gene"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
1981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
2041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
2101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
2161 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc tgaacgatgg agcggagaat
2221 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
2281 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
2341 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
2401 ctggggactt tccacaccct aactgacaca cattccacag cggatccgtc gaccgatgcc
2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
2941 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
3181 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
4921 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca
4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
5221 tgcaagtgca ggtgccagaa catttctcta tcgata
