-
平臺編號：bio-114931
-
質粒信息： pShuttle-CMV
-
規(guī)格：2ml甘油管 20ul質粒
-
用途：/
-
服務費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pShuttle-CMV
| 啟動子 | CMV |
| 復制子 | pUC |
| 終止子 | SV40 poly(A) signal |
| 質粒大小 | 7384bp |
| 原核抗性 | Kan |
| 篩選標記 | G418 |
| 克隆菌株 | Stbl3 |
| 培養(yǎng)條件 | 37度 |
| 表達宿主 | 哺乳細胞 |
| 誘導方式 | 無須誘導，瞬時表達 |
| 5'測序引物 | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3'測序引物 | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
| 載體宿主 | 哺乳細胞,腺病毒 |
| 載體用途 | 蛋白表達 |
| 基因類型 | ORF |
| 原核抗性 | Kan |
| 真核抗性 | Neo/G418 |
| 質粒簡介 | The vector pShuttle-CMV contains a multiple cloning site sandwiched between the CMV promoter and the SV40 polyadenylation signal and is suitable for insertion of a large cDNA (up to 6.6 kb). pShuttle contains only a multiple cloning site. This allows for the insertion of an entire expression cassette, including specialized promoters and termination signals (up to 7.5 kb). The regions indicated as arms are the stretches of sequence homology with pAdEasy-1 where the homologous recombination occurs. The R-ITR and L-ITR regions are short inverted terminal repeats (Left and Right) which have a role in replication of the viral DNA. |
質粒序列
LOCUS Exported 7384 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7384)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7384
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 412..715
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 716..919
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal 1130..1251
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4756..5344)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 6155..6949
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
gap 7364..7384
/estimated_length="21"
ORIGIN
1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa
61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag
121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa
181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta
241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa
301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannnnta
361 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
541 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
721 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
781 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
841 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
901 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat ctggtaccgt
961 cgacgcggcc gctcgagcct aagcttctag ataagatatc cgatccaccg gatctagata
1021 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
1081 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
1141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
1201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcnnnnt
1261 aagggtggga aagaatatat aaggtggggg tcttatgtag ttttgtatct gttttgcagc
1321 agccgccgcc gccatgagca ccaactcgtt tgatggaagc attgtgagct catatttgac
1381 aacgcgcatg cccccatggg ccggggtgcg tcagaatgtg atgggctcca gcattgatgg
1441 tcgccccgtc ctgcccgcaa actctactac cttgacctac gagaccgtgt ctggaacgcc
1501 gttggagact gcagcctccg ccgccgcttc agccgctgca gccaccgccc gcgggattgt
1561 gactgacttt gctttcctga gcccgcttgc aagcagtgca gcttcccgtt catccgcccg
1621 cgatgacaag ttgacggctc ttttggcaca attggattct ttgacccggg aacttaatgt
1681 cgtttctcag cagctgttgg atctgcgcca gcaggtttct gccctgaagg cttcctcccc
1741 tcccaatgcg gtttaaaaca taaataaaaa accagactct gtttggattt ggatcaagca
1801 agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg taggcccggg accagcggtc
1861 tcggtcgttg agggtcctgt gtattttttc caggacgtgg taaaggtgac tctggatgtt
1921 cagatacatg ggcataagcc cgtctctggg gtggaggtag caccactgca gagcttcatg
1981 ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa
2041 aatgtctttc agtagcaagc tgattgccag gggcaggccc ttggtgtaag tgtttacaaa
2101 gcggttaagc tgggatgggt gcatacgtgg ggatatgaga tgcatcttgg actgtatttt
2161 taggttggct atgttcccag ccatatccct ccggggattc atgttgtgca gaaccaccag
2221 cacagtgtat ccggtgcact tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa
2281 gaacttggag acgcccttgt gacctccaag attttccatg cattcgtcca taatgatggc
2341 aatgggccca cgggcggcgg cctgggcgaa gatatttctg ggatcactaa cgtcatagtt
2401 gtgttccagg atgagatcgt cataggccat ttttacaaag cgcgggcgga gggtgccaga
2461 ctgcggtata atggttccat ccggcccagg ggcgtagtta ccctcacaga tttgcatttc
2521 ccacgctttg agttcagatg gggggatcat gtctacctgc ggggcgatga agaaaacggt
2581 ttccggggta ggggagatca gctgggaaga aagcaggttc ctgagcagct gcgacttacc
2641 gcagccggtg ggcccgtaaa tcacacctat taccgggtgc aactggtagt taagagagct
2701 gcagctgccg tcatccctga gcaggggggc cacttcgtta agcatgtccc tgactcgcat
2761 gttttccctg accaaatccg ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa
2821 ggaagcaaag tttttcaacg gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg
2881 accaagcagt tccaggcggt cccacagctc ggtcacctgc tctacggcat ctcgatccag
2941 catatctcct cgtttcgcgg gttggggcgg ctttcgctgt acggcagtag tcggtgctcg
3001 tccagacggg ccagggtcat gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg
3061 gtcacggtga aggggtgcgc tccgggctgc gcgctggcca gggtgcgctt gaggctggtc
3121 ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc
3181 atggtgtcat agtccagccc ctccgcggcg tggcccttgg cgcgcagctt gcccttggag
3241 gaggcgccgc acgaggggca gtgcagactt ttgagggcgt agagcttggg cgcgagaaat
3301 accgattccg gggagtaggc atccgcgccg caggccccgc agacggtctc gcattccacg
3361 agccaggtga gctctggccg ttcggggtca aaaaccaggt ttcccccatg ctttttgatg
3421 cgtttcttac ctctggtttc catgagccgg tgtccacgct cggtgacgaa aaggctgtcc
3481 gtgtccccgt atacagactn nngtttaaac gaattcnnna tataaaatgc aaggtgctgc
3541 tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca
3601 gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca
3661 tgtctgcggg tttctgcata aacacaaaat aaaataacaa aaaaacattt aaacattaga
3721 agcctgtctt acaacaggaa aaacaaccct tataagcata agacggacta cggccatgcc
3781 ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc accaccgaca gctcctcggt
3841 catgtccgga gtcataatgt aagactcggt aaacacatca ggttgattca tcggtcagtg
3901 ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta
3961 cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa
4021 aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac
4081 agcggcagcc taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac
4141 tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta
4201 tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc
4261 acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact
4321 tccgttttcc cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac
4381 aagttactcc gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca
4441 caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat attattgatg
4501 atnnnttaat taaggatccn nncggtgtga aataccgcac agatgcgtaa ggagaaaata
4561 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
4621 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
4681 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
4741 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
4801 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
4861 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
4921 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
4981 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
5041 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
5101 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
5161 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
5221 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
5281 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
5341 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
5401 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
5461 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
5521 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
5581 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
5641 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
5701 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
5761 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
5821 tgnnnnaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg
5881 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
5941 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
6001 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
6061 ctgcaaagta aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag
6121 ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
6181 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
6241 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
6301 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
6361 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
6421 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
6481 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
6541 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
6601 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
6661 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
6721 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
6781 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
6841 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
6901 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttttgttaaa
6961 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaac atcccttata
7021 aatcaaaaga atagaccgcg atagggttga gtgttgttcc agtttggaac aagagtccac
7081 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
7141 cactacgtga accatcaccc aaatcaagtt ttttgcggtc gaggtgccgt aaagctctaa
7201 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
7261 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
7321 tcacgctgcg cgtaaccacc acacccgcgc gcttaatgcg ccgnnnnnnn nnnnnnnnnn
7381 nnnn
