| 載體名稱 | pGL4.43 ; pGL4.43[luc2P/XRE/Hygro] |
| 質(zhì)粒類型 | 信號通路報告載體；哺乳動物載體；螢火蟲熒光素酶報告載體 |
| 高拷貝/低拷貝 | -- |
| 克隆方法 | 限制性內(nèi)切酶，多克隆位點 |
| 啟動子 | Minimal Promotor |
| 載體大小 | 6067 bp |
| 5' 測序引物及序列 | -- |
| 3' 測序引物及序列 | -- |
| 載體標(biāo)簽 | luc2P |
| 載體抗性 | 氨芐青霉素 |
| 篩選標(biāo)記 | 潮霉素(Hygromycin) |
| 克隆菌株 | TOP10等常規(guī)菌株 |
| 宿主細(xì)胞（系） | HEK293等 |
| 備注 | pGL4.43[luc2P/XRE/Hygro]載體是信號通路報告載體；含異己物應(yīng)答元件XRE；含螢火蟲熒光素酶報告基因luc2P。 |
| 穩(wěn)定性 | 穩(wěn)表達(dá) |
| 組成型/誘導(dǎo)型 | 組成型 |
| 病毒/非病毒 | 非病毒 |
| pGL4.43載體簡介 | The pGL4.43[luc2P/XRE/Hygro] Vector contains three copies of a xenobiotic response element (XRE) that drives transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence, which allows luc2P protein levels to respond more quickly than those of luc2 to induction of transcription. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a gene for hygromycin resistance to allow selection of stably transfected mammalian cell lines. |
