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平臺編號：bio-69212
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質(zhì)粒信息： pJMP1
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規(guī)格：2ml甘油管 20ul質(zhì)粒
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用途：/
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pJMP1
| 別稱 | Plasmid#79873;pAX01-dCas9 |
| 啟動子 | xylA |
| 原核抗性 | Amp |
| 篩選標記 | Ery |
| 克隆菌株 | DH5a |
| 培養(yǎng)條件 | 37度 |
| 表達宿主 | 枯草桿菌 |
| 載體宿主 | 枯草桿菌 |
| 載體用途 | 基因敲除 |
| 原核抗性 | Amp |
| 質(zhì)粒簡介 | pJMP1質(zhì)粒是一個枯草桿菌基因編輯質(zhì)粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。 A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Journal |
