啟動子 | CMV |
復(fù)制子 | pUC |
質(zhì)粒大小 | 7567bp |
原核抗性 | Amp |
篩選標(biāo)記 | EGFP |
克隆菌株 | Stbl3 |
培養(yǎng)條件 | 37度 |
載體宿主 | 哺乳細(xì)胞,慢病毒 |
載體用途 | 干擾沉默 |
基因類型 | shRNA |
原核抗性 | Amp |
熒光蛋白 | 綠色 |
質(zhì)粒簡介 |
EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
pSico shRNA cloning protocol |