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平臺(tái)編號(hào)：bio-83396
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拉丁屬名： pKR1B-LAC4-1
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用途：ATCC原裝進(jìn)口
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
pKR1B-LAC4-1*
pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
(ATCC? 20758?)
Designations pKR1B-LAC4-1 [L1582/pKR1B-LAC4-1, YNN27/pKR1B-LAC4-1]
GenBank Definition Yeast (K.lactis) beta-galactosidase gene, 5' end
GenBank Number M18108
Species Kluyveromyces lactis (Dombrowski) van der Walt
Depositors University of Kentucky, RC Dickson, University of Kentucky
Applications produces protein beta-galactosidase beta-D-galactosidase produces protein lactose permease This is a Lac+ strain expressing K. lactis lactose permease and beta-galactosidase.
Vector Construct size (kb): 20.0 Insert DNA: genomic
Insert lengths(kb): 12.0 Gene product: beta-galactosidase(beta-D-galactosidase) [LAC4] Target Gene: lactose permease, beta-galactosidase Insert Size (kb) 12.0
Genotype MATalpha trp1-289 ura3-52 gal2
Media ATCC? Medium 1245: YEPD
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments The plasmid contains the following restriction sites (approximate kb from the pBR322-derived EcoRI site): XhoI--2.5; EcoRI--8.1, 9.1, 11.4, 11.9, 12.6, 15.1, 16; BamHI--14.6, 19.7. Fermentation of lactose in whey
GenBank M18108 contains the sequence for the promoter region and part of the coding sequence for LAC4. The vector is a derivative of pBR322 carrying the kanamycin (G418) resistance gene of Tn903 and ARS1B from Kluyveromyces lactis. This plasmid can replicate in E. coli, S. cerevisiae, and K. lactis. The order of the major features in this plasmid is: ampR - kanR - LAC12 - LAC4 > - ARS1B.
References Sreekrishna K, et al. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28: 73-81, 1984. PubMed: 6329916
Sreekrishna K, Dickson RC. Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc. Natl. Acad. Sci. USA 82: 7909-7913, 1985. PubMed: 3934664
Leonardo JM, et al. Identification of upstream activator sequences that regulate induction of the beta-galactosidase gene in Kluyveromyces lactis. Mol. Cell. Biol. 7: 4369-4376, 1987. PubMed: 3125422
Dickson RC, Sreekrishna KK. Lac+ Saccharomyces cerevisiae.
US Patent 5,047,340 dated Sep 10 1991
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
