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Name:
Sphingobium chlorophenolicum (Nohynek et al. 1996) Takeuchi et al. 2001
Synonym(s):
Sphingomonas chlorophenolica Nohynek et al. 1996
DSM No.:
7098, Type strain
Other collection no.
or WDCM no.:
ATCC 33790,CIP 104885,IFO 16172,NBRC 16172
Isolated from:
soil; pentachlorophenol contaminated
Country:
United States of America
Date of sampling:
before 15.06.1992
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- ATCC <- R.U. Edgehill (Arthrobacter sp.)
Cultivation conditions:
Medium 457 with 0.3 g/l glucose, glycerol and Na-succinate, each and 66 mg/l pentachlorophenol, 30°C
or
Medium 53 , 30°C
or
Medium 1 , Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates, 30°C

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates of the three media mentioned

Complete DSMZ Media List
Summary and
additional information:
<- ATCC <- R.U. Edgehill (Arthrobacter sp.). Soil; pentachlorophenol contaminated; USA. Type strain. Taxonomy/description (4692, 6429, 6462, 6485, 8160). Sequence accession no. X87161. 4 Plasmids <50, 50, 160 and 200 kb in size. Produces pentachlorophenol-dehalogenase (4694). Utilizes pentachlorophenol (4692), 2,4,6-trichlorophenol (4692). Soil bioremediation (4693). (Medium 457 with 0.3 g/l glucose, glycerol and Na-succinate, each and 66 mg/l pentachlorophenol, 30°C or Medium 1, 30°C, Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates or Medium 53, 30°C). Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates of the three media mentioned.
Literature:
4692, 4693, 4694, 6429, 6462, 6485, 8160, 25439
Risk group:
1 (classification according to German TRBA)
Supplied as:
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Price Category for this culture: 1
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