Name:
Cupriavidus necator Makkar and Casida 1987
DSM No.:
13439
Strain designation:
H850-4LT
Isolated from:
strain H850 (NRRL B-15940) genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.
Country:
country of origin unknown
H850 was isolated from Hudson River dredge, USA
Date of sampling:
before 12.04.2000
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- R M. Wittich, GBF, genetic modification <- NRRL
Cultivation conditions:
Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 28°C
or
Medium 1a , 28°C
Medium/media recipe(s) required for preparing 1a:
- Medium 1
Complete DSMZ Media List
Summary and
additional information:
<- R M. Wittich, GBF, genetic modification <- NRRL. (Wautersia eutropha).Strain H850 (NRRL B-15940) was genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase xylXYZ, of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.; H850 was isolated from Hudson River dredge soil, USA. GMO of H850 (NRRL B-15940). Utilizes monochlorinated benzoates, 3,5-dichlorobenzoate and polychlorinated biphenyls (PCBs) such as Arochlor 1221 or 1232 (13435). (Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 20-30°C or Medium 1a, 20-30°C).
Literature:
13435
Risk group:
1 (classification according to German TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz), Category C
Supplied as:
Delivery form Prices
Freeze Dried
Active culture on request
DNA
Price Category for this culture: 1
Freight and handling charges will be added. See price list.
DSM13439
Cupriavidus necator Makkar and Casida 1987
DSM No.:
13439
Strain designation:
H850-4LT
Isolated from:
strain H850 (NRRL B-15940) genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.
Country:
country of origin unknown
H850 was isolated from Hudson River dredge, USA
Date of sampling:
before 12.04.2000
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- R M. Wittich, GBF, genetic modification <- NRRL
Cultivation conditions:
Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 28°C
or
Medium 1a , 28°C
Medium/media recipe(s) required for preparing 1a:
- Medium 1
Complete DSMZ Media List
Summary and
additional information:
<- R M. Wittich, GBF, genetic modification <- NRRL. (Wautersia eutropha).Strain H850 (NRRL B-15940) was genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase xylXYZ, of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.; H850 was isolated from Hudson River dredge soil, USA. GMO of H850 (NRRL B-15940). Utilizes monochlorinated benzoates, 3,5-dichlorobenzoate and polychlorinated biphenyls (PCBs) such as Arochlor 1221 or 1232 (13435). (Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 20-30°C or Medium 1a, 20-30°C).
Literature:
13435
Risk group:
1 (classification according to German TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz), Category C
Supplied as:
Delivery form Prices
Freeze Dried
Active culture on request
DNA
Price Category for this culture: 1
Freight and handling charges will be added. See price list.
DSM13439
