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Secundilactobacillus paracollinoides (Suzuki et al. 2004) Zheng et al. 2020
Taxonomy in NCBI database: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae.
11969T <-- K. Suzuki LA2.
Accessioned in 2003.
=BCRC 80778 =CIP 108394 =DSM 15502 =KCTC 25259 =LMG 22473 =VTT E-042481.
Lactobacillus paracollinoides.
Type strain [5683,12937].
Medium: 426;  Temperature: 25°C; Anaerobic.

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Source: Brewery environment [5683].
Biochemistry/Physiology: [5683,7492].
G+C (mol%): 44.8 (HPLC) [5683], 46.8 (genome sequence) [12937].
DNA-DNA relatedness: [5683,7492].
Phylogeny: 16S rRNA gene (E16651, LC483558) [5683].
DNA typing: Ribotyping [7492].
Other taxonomic data: Beer-spoilage ability [5683,7492]; Specific PCR primers [5959,7492,7493]; Hop resistance [7563]; Rapid detection [7763].
Taxonomy: [5960].
Genome sequence: AZFD00000000 [12937], BBAG00000000.
NCBI Taxonomy ID: 240427.
Genomic DNA is available from RIKEN BRC-DNA Bank: JGD 12543.
Publication(s) using this strain [B04084, A07246, A07251, A10149, A10316, B11210, B13281, B13387, B15270, B17320].
Patent publication(s) using this strain [WO2007/111210, JP2008-263971A, JP2010-081935A].
 Related information on delivery / use of the strain
Biosafety level 1
Terms and conditions Not applicable
Export control (1) No
Distribution control in Japan (2) No
Genetically modified microorganism No
Technical information Use Beer medium for cultivation because the beer-spoilage ability may be lost by culturing on other media. Preserve this strain by freezing at -80°C because the beer-spoilage ability gene is lost by freeze-drying.
Additional information -
 (1) in complying with the Foreign Exchange and Foreign Trade Control Law of Japan
 (2) in complying with the Plant Protection Law of Japan

 Delivery category
Domestic B (Actively growing culture of a microbial strain that is not preserved as a dried culture / a microbial strain of which dried ampoules are not available)
Overseas B (Actively growing culture of a microbial strain that is not preserved as a dried culture / a microbial strain of which dried ampoules are not available)
Viability and purity assays of this product were performed at the time of production as part of quality control. The authenticity of the culture was confirmed by analyzing an appropriate gene sequence, e.g., the 16S rRNA gene for prokaryotes, the D1/D2 region of LSU rRNA gene, the ITS region of the nuclear rRNA operon, etc. for eukaryotes. The characteristics and/or functions of the strain appearing in the catalogue are based on information from the corresponding literature and JCM does not guarantee them.