Species:Escherichia coli
Group:K12
Strain designation:BW313
Accession number:LMBP 1325
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6796
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:λ-ung-1 relA1 dut-1 spoT1 thiE1(Source:CGSC 6796)
Phenotype:-
Properties:Use of this strain is required for the preparation of single-stranded phage M13 or phasmid DNA to be used in the site-directed mutagenesis method of Kunkel(Kunkel,1985).
Further information:-
Original reference:Kunkel,Proc.Natl.Acad.Sci.U.S.A.82(1985),488-492[PMID:3881765]
Related reference:Warner et al.,J.Bacteriol.145(1981),687-695[PMID:6109711]
Duncan and Weiss,J.Bacteriol.151(1982),750-755[PMID:7047496]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 BW313(LMBP 1325)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Kunkel,1985.
Group:K12
Strain designation:BW313
Accession number:LMBP 1325
Depositor:Prof.Dr E.Remaut1 2
1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium
2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Other culture collection numbers:CGSC 6796
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:λ-ung-1 relA1 dut-1 spoT1 thiE1(Source:CGSC 6796)
Phenotype:-
Properties:Use of this strain is required for the preparation of single-stranded phage M13 or phasmid DNA to be used in the site-directed mutagenesis method of Kunkel(Kunkel,1985).
Further information:-
Original reference:Kunkel,Proc.Natl.Acad.Sci.U.S.A.82(1985),488-492[PMID:3881765]
Related reference:Warner et al.,J.Bacteriol.145(1981),687-695[PMID:6109711]
Duncan and Weiss,J.Bacteriol.151(1982),750-755[PMID:7047496]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 BW313(LMBP 1325)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Kunkel,1985.
