Species:Escherichia coli
Group:K12
Strain designation:IM30B
Accession number:LMBP 9583
Depositor:Dr I.Monk1
1 Department of Microbiology and Immunology,Doherty Institute,University of Melbourne,Melbourne,Australia
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:mcrAΔ(mrr-hsdRMS-mcrBC)φ80lacZΔM15ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL endA1 nupGΔdcmΩPhelp-hsdMS(CC30-2)ΩPN25-hsdS(CC30-1)
Phenotype:StrR
Properties:This bacterial host strain was derived from E.coli K12 DH10B by deleting the dcm gene encoding cytosine methylation.Additionally,the hsdMS genes encoding methylase and specificity genes(from Staphylococcus aureus clonal complex 30-MRSA252)were introduced onto the chromosome at neutral locations via recombineering.The strain can be transformed efficiently with large plasmids due to deoR(nupG)mutation.Plasmids isolated from this strain transform S.aureus clonal complex 30-MRSA252 at high efficiency.
Further information:-
Original reference:Monk et al.,MBio 6(2015),e00308-15[PMID:26015493][DOI:10.1128/mBio.00308-15]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 IM30B(LMBP 9583)is available at BCCM/GeneCorner.It was deposited by Dr I.Monk and was published in Monk et al.,2015.
Group:K12
Strain designation:IM30B
Accession number:LMBP 9583
Depositor:Dr I.Monk1
1 Department of Microbiology and Immunology,Doherty Institute,University of Melbourne,Melbourne,Australia
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:mcrAΔ(mrr-hsdRMS-mcrBC)φ80lacZΔM15ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL endA1 nupGΔdcmΩPhelp-hsdMS(CC30-2)ΩPN25-hsdS(CC30-1)
Phenotype:StrR
Properties:This bacterial host strain was derived from E.coli K12 DH10B by deleting the dcm gene encoding cytosine methylation.Additionally,the hsdMS genes encoding methylase and specificity genes(from Staphylococcus aureus clonal complex 30-MRSA252)were introduced onto the chromosome at neutral locations via recombineering.The strain can be transformed efficiently with large plasmids due to deoR(nupG)mutation.Plasmids isolated from this strain transform S.aureus clonal complex 30-MRSA252 at high efficiency.
Further information:-
Original reference:Monk et al.,MBio 6(2015),e00308-15[PMID:26015493][DOI:10.1128/mBio.00308-15]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 IM30B(LMBP 9583)is available at BCCM/GeneCorner.It was deposited by Dr I.Monk and was published in Monk et al.,2015.
