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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:JK242

　　Accession number:LMBP 917

　　Depositor:Prof.Dr R.Beyaert1 2 via Prof.Dr E.Remaut1 2.It was originally obtained from Dr J.Parker3

　　1 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　2 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium

　　3 Department of Microbiology,Southern Illinois University,Illinois,USA

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox+L-asparagine(100μg/ml)

　　Selection marker:streptomycin(25μg/ml)

　　Temperature:37°C

　　Genotype:asnA31 asnB32 thi-1 relA spoT rpsL125(source:Parker et al.,1983)

　　Phenotype:StrR

　　Properties:The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene.It carries also the streptomycin-resistant allele,rpsL,a mutation leading to increased translational accuracy(Parker et al.,1983).

　　Further information:The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.

　　Original reference:Parker et al.,Mol.Gen.Genet.180(1980),275-281[PMID:6780757]

　　Related reference:Felton et al.,J.Bacteriol.142(1980),221-228[PMID:6102983]

　　Parker et al.,J.Biol.Chem.258(1983),10007-10012[PMID:6885754]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli JK242(LMBP 917)is available at BCCM/GeneCorner.It was deposited by Prof.Dr R.Beyaert via Prof.Dr E.Remaut and was published in Parker et al.,1980.