Species:Escherichia coli
Group:K12
Strain designation:JK242
Accession number:LMBP 917
Depositor:Prof.Dr R.Beyaert1 2 via Prof.Dr E.Remaut1 2.It was originally obtained from Dr J.Parker3
1 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
2 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
3 Department of Microbiology,Southern Illinois University,Illinois,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox+L-asparagine(100μg/ml)
Selection marker:streptomycin(25μg/ml)
Temperature:37°C
Genotype:asnA31 asnB32 thi-1 relA spoT rpsL125(source:Parker et al.,1983)
Phenotype:StrR
Properties:The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene.It carries also the streptomycin-resistant allele,rpsL,a mutation leading to increased translational accuracy(Parker et al.,1983).
Further information:The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Parker et al.,Mol.Gen.Genet.180(1980),275-281[PMID:6780757]
Related reference:Felton et al.,J.Bacteriol.142(1980),221-228[PMID:6102983]
Parker et al.,J.Biol.Chem.258(1983),10007-10012[PMID:6885754]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli JK242(LMBP 917)is available at BCCM/GeneCorner.It was deposited by Prof.Dr R.Beyaert via Prof.Dr E.Remaut and was published in Parker et al.,1980.
Group:K12
Strain designation:JK242
Accession number:LMBP 917
Depositor:Prof.Dr R.Beyaert1 2 via Prof.Dr E.Remaut1 2.It was originally obtained from Dr J.Parker3
1 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium
2 VIB-UGent Center for Inflammation Research,VIB,Ghent,Belgium
3 Department of Microbiology,Southern Illinois University,Illinois,USA
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox+L-asparagine(100μg/ml)
Selection marker:streptomycin(25μg/ml)
Temperature:37°C
Genotype:asnA31 asnB32 thi-1 relA spoT rpsL125(source:Parker et al.,1983)
Phenotype:StrR
Properties:The JK242 strain is auxotrophic for asparagine because of the inactivated asnA and asnB gene.It carries also the streptomycin-resistant allele,rpsL,a mutation leading to increased translational accuracy(Parker et al.,1983).
Further information:The streptomycin resistance has been experimentally confirmed by BCCM/GeneCorner.
Original reference:Parker et al.,Mol.Gen.Genet.180(1980),275-281[PMID:6780757]
Related reference:Felton et al.,J.Bacteriol.142(1980),221-228[PMID:6102983]
Parker et al.,J.Biol.Chem.258(1983),10007-10012[PMID:6885754]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli JK242(LMBP 917)is available at BCCM/GeneCorner.It was deposited by Prof.Dr R.Beyaert via Prof.Dr E.Remaut and was published in Parker et al.,1980.
