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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:3000

　　Accession number:LMBP 1393

　　Depositor:Prof.Dr E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:37°C

　　Genotype:HfrH relA1 spoT1 thi-1

　　Phenotype:-

　　Properties:Useful for propagation of F'specific phages.

　　Further information:-

　　Original reference:Loomis et al.,J.Mol.Biol.23(1967),487-494[PMID:5340246]

　　Related reference:Bachmann,in'Escherichia coli and Salmonella:Cellular and Molecular Biology',American Society for Microbiology,Washington DC,2nd edition(1996),2460-2488[ISSN/ISBN:1555810845]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 3000(LMBP 1393)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut and was published in Loomis et al.,1967.