亚日韩在线中文字幕亚洲,国产在线播放鲁啊鲁视频,张柏芝手扒性器全部图片,亚洲成无码电影在线观看

　　Species:Escherichia coli

　　Group:K12

　　Strain designation:M5219

　　Accession number:LMBP 130

　　Depositor:Prof.E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:28°C

　　Genotype:lacZam trpAam rpsLλ(bio252 cI857ΔH1)

　　Phenotype:StrR

　　Properties:Expression host for plasmids containing phageλ's PL or PR promoter.Induction is obtained by shifting the culture from 28°C to 42°C.The residentλis a defective lysogen.ΔH1 removes part of cro and all genes to the right of it.bio252 removes all genes to the left of cIII.cI857 is ind-.At 42°C,N is expressed from the lysogen.As a consequence,transcription termination signals that might be present downstream from the PL promoter and the nutL site,as present on the pPL-series of plasmids,can be overridden.

　　Further information:Useful for the expression of cloned genes carrying transcription terminators between the start of the gene and the insertion point.

　　Original reference:Remaut et al.,Gene 15(1981),81-93[PMID:6271633]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 M5219(LMBP 130)is available at BCCM/GeneCorner.It was deposited by Prof.E.Remaut and was published in Remaut et al.,1981.