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　　Species:Escherichia coli

　　Group:K12xB

　　Strain designation:DB3.1

　　Accession number:LMBP 4098

　　Depositor:Life Technologies Corporation1

　　1 Waltham,Massachusetts,USA

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:streptomycin(25μg/ml)

　　Temperature:37°C

　　Genotype:gyrA462 endA1Δ(sr1-recA)mcrB mrr hsdS20 glnV44(=supE44)ara14 galK2 lacY1 proA2 rpsL20 xyl5 leuB6 mtl1

　　Phenotype:StrR rB-mB-

　　Properties:DB3.1 is a HB101 derivative containing the gyrA462 allele which renders the strain resistant to the toxic effects of the ccdB gene.

　　Further information:Original Invitrogen catalogue number:11782-018.However,this strain is no longer available at Invitrogen.

　　Alternative:ccdB-resistant One Shot ccdB Survival 2 T1R Competent Cells,of which the ccdB-resistance mechanism is not known;it is based on empirical results and the success or failure of the transformation of the cells is plasmid-dependent(personal communication Invitrogen).

　　Original reference:-

　　Related reference:Bernard et al.,J.Mol.Biol.226(1992),735-745[PMID:1324324]

　　Restricted use:-BCCM MTA adapted by Life Technologies Corporation

　　Refer in your Materials and Methods:

　　Escherichia coli K12xB DB3.1(LMBP 4098)is available at BCCM/GeneCorner.It was deposited by Life Technologies Corporation.