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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:DC10B

　　Accession number:LMBP 9585

　　Depositor:Dr I.Monk1

　　1 Department of Microbiology and Immunology,Doherty Institute,University of Melbourne,Melbourne,Australia

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:37°C

　　Genotype:mcrA?(mrr-hsdRMS-mcrBC)?80lacZ?M15?lacX74 recA1 araD139?(ara-leu)7697 galU galK rpsL endA1 nupG?dcm

　　Phenotype:StrR

　　Properties:This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E.coli K12 DH10B.It is a universal Staphylococcal cloning host.

　　Further information:-

　　Original reference:Monk et al.,MBio 3(2012),e00277-11[PMID:22434850][DOI:10.1128/mBio.00277-11]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 DC10B(LMBP 9585)is available at BCCM/GeneCorner.It was deposited by Dr I.Monk and was published in Monk et al.,2012.