Species:Escherichia coli
Group:K12
Strain designation:DC10B
Accession number:LMBP 9585
Depositor:Dr I.Monk1
1 Department of Microbiology and Immunology,Doherty Institute,University of Melbourne,Melbourne,Australia
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:mcrA?(mrr-hsdRMS-mcrBC)?80lacZ?M15?lacX74 recA1 araD139?(ara-leu)7697 galU galK rpsL endA1 nupG?dcm
Phenotype:StrR
Properties:This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E.coli K12 DH10B.It is a universal Staphylococcal cloning host.
Further information:-
Original reference:Monk et al.,MBio 3(2012),e00277-11[PMID:22434850][DOI:10.1128/mBio.00277-11]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 DC10B(LMBP 9585)is available at BCCM/GeneCorner.It was deposited by Dr I.Monk and was published in Monk et al.,2012.
Group:K12
Strain designation:DC10B
Accession number:LMBP 9585
Depositor:Dr I.Monk1
1 Department of Microbiology and Immunology,Doherty Institute,University of Melbourne,Melbourne,Australia
Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).
Medium:LB-Lennox
Selection marker:/
Temperature:37°C
Genotype:mcrA?(mrr-hsdRMS-mcrBC)?80lacZ?M15?lacX74 recA1 araD139?(ara-leu)7697 galU galK rpsL endA1 nupG?dcm
Phenotype:StrR
Properties:This bacterial host strain is a DNA cytosine methyltransferase negative mutant of E.coli K12 DH10B.It is a universal Staphylococcal cloning host.
Further information:-
Original reference:Monk et al.,MBio 3(2012),e00277-11[PMID:22434850][DOI:10.1128/mBio.00277-11]
Restricted use:-BCCM MTA
Refer in your Materials and Methods:
Escherichia coli K12 DC10B(LMBP 9585)is available at BCCM/GeneCorner.It was deposited by Dr I.Monk and was published in Monk et al.,2012.
