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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:DH5αF

　　Accession number:LMBP 1596

　　Depositor:Prof.Dr E.Remaut1 2

　　1 Department for Molecular Biomedical Research,VIB,Ghent,Belgium

　　2 Department of Biomedical Molecular Biology,Ghent University,Ghent,Belgium

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Lennox

　　Selection marker:/

　　Temperature:37°C

　　Genotype:F+Δ(argF-lac)169φ80dlacZ58(M15)ΔphoA8 glnX44(AS)λ-deoR481 rfbC1 gyrA96 recA1 endA1 thiE1 hsdR17

　　Phenotype:NalR rK-mK+

　　Properties:Useful strain for Lacαcomplementation.This strain does not contain the lacIq gene and therefore repression on the lac promoter,present on high-copy plasmids,is incomplete.Consequently,this strain does not require IPTG to induce expression from the lac promoter even though it expresses the Lac repressor.The copy number of most plasmids exceeds the repressor number in the cells.If there are concerns about obtaining maximal levels of expression,add IPTG to a final concentration of 1 mM.

　　Further information:This strain contains the F factor of NK3 and therefore can be used for infection with M13 or fd phages and derivatives.

　　Original reference:-

　　Related reference:Hanahan,J.Mol.Biol.166(1983),557-580[PMID:6345791]

　　Grant et al.,Proc.Natl.Acad.Sci.U.S.A.87(1990),4645-4649[PMID:2162051]

　　Woodcock et al.,Nucleic Acids Res.17(1989),3469-3478[PMID:2657660]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 DH5αF(LMBP 1596)is available at BCCM/GeneCorner.It was deposited by Prof.Dr E.Remaut.