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　　Species:Escherichia coli

　　Group:K12

　　Strain designation:DH5αpir116 variant

　　Accession number:LMBP 7961

　　Depositor:Prof.Dr M.Herrington1

　　1 Biology Dept,Concordia University,Montreal,Canada

　　It was obtained from Dr G.Philips2

　　2 College of Veterinary Medicine,Dept of Microbiology,Iowa State University,Ames,Iowa,USA

　　Containment level:This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms,and its updates(see also the Belgian risk group classification).

　　Medium:LB-Miller

　　Selection marker:/

　　Temperature:37°C

　　Genotype:endA1 hsdR17 glnV44(=supE44)thi-1 recA1 gyrA96 relA1Φ80dlacΔ(lacZ)M15Δ(lacZYA-argF)U169 zdg-232::Tn10 uidA::pir116

　　Phenotype:TetS NalS

　　Properties:This is a host strain for R6Kγori plasmids.In pir116 strains,R6K-containing plasmids replicate like high-copy pUC plasmids.

　　Further information:Personal communications of Prof.Dr M.Herrington:The E.coli K12 DH5αpir116 variant appears to have lost the Tn10 and is consequently tetracycline sensitive.

　　BCCM/GeneCorner could not experimentally confirm the nalidixic acid resistance,conferred by gyrA96,nor the tetracycline resistance,conferred by Tn10.

　　Original reference:Platt et al.,Plasmid 43(2000),12-23[PMID:10610816]

　　Related reference:Sitaras et al.,Plasmid 65(2011),232-238[PMID:21376749][DOI:10.1016/j.plasmid.2011.02.005]

　　Grant et al.,Proc.Natl.Acad.Sci.U.S.A.87(1990),4645-4649[PMID:2162051]

　　Restricted use:-BCCM MTA

　　Refer in your Materials and Methods:

　　Escherichia coli K12 DH5αpir116 variant(LMBP 7961)is available at BCCM/GeneCorner.It was deposited by Prof.Dr M.Herrington and was published in Platt et al.,2000.